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KMID : 1041220130510020137
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Journal of Sericultural and Entomological Science
2013 Volume.51 No. 2 p.137 ~ p.141
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Comparison of scanning electron microscopic structures and nucleotide sequences variation of ITS1, 5.8S ribosomal RNA gene and ITS2 region in three Peruvian entomopathogenic fungal isolates
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Han Sang-Hoon
Nam Sung-Hee
Lee Heui-Sam
Yeo Joo-Hong
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Abstract
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In this study, nucleotide sequence structures of intergenic transcribed spacer (ITS) 1, complete 5.8S ribosomal RNA gene and ITS 2 region were analyzed to identify three Peruvian entomopathogenic fungal isolates. The isolates had highly conserved sequence region in 5.8S rRNA gene and unique sequences in ITS 1 and 2 region among them. 5.8S rRNA gene regions were highly conserved and showed high homoloies among tested isolates. In contrast, ITS region showed species-specific sequence region, resulting in inter-genus differencies. Scanning electron microscopic images of these isolates supported the result of ITS-based identification. From these result, Peruvian entomopathogenic fungal isolate J270, J278, were identified as Beauveria bassiana and J271 was identified as Lecanicillium attenuatum.
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KEYWORD
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Beauveria bassiana, Lecanicillium attenuatum, Intergenic transcribed spacer, Peruvian entomopathogenic fungi, Bioinsecticide
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